孵化场的朋友看过来
本帖最后由 常老师1 于 2021-11-3 17:20 编辑最近一直在研究孵化场死胚带菌情况。通过研究发现，早期死亡比较多的种蛋，鸡胚带菌情况比较严重。反之，大部分发育成熟的死胚中细菌不多！
最近分析了一家著名蛋种鸡场的30个死胚，只有一个臭蛋、一个早期死亡胚蛋。其余的都已发育成熟，只是脱水比较严重（可能的死亡原因）。
细菌分离培养结果，一个含有魏氏梭菌，一个含有大肠杆菌。
http://magimg.jbzyw.com/pic/20211103/1635928361671560_945.jpg
估计是带菌多的没等发育成就死胚了 看来教授又没事干了。怎么想起来研究这个了？
这些事别人早就研究透了，不用再研究了，去查查资料就行了。 有点废话了，鸡蛋里面这么多抗体和溶菌酶。除非那个鸡蛋不正常，因为不正常所以也发育不了。 您肯定也分出过沙门氏菌吧 常老师你好，我觉得你这样设计实验的话，实验结果很难说明问题。
要想分析受精蛋可能的污染问题（或者是造成死胚和弱雏的原因），需要对受精蛋和未受精蛋同时进行孵化处理，在19-21天内进行采样分菌，另外也要对鸡舍污染物（如粪便）、死胚、孵化出来1-7内死亡雏鸡的肝脏和卵黄囊进行采样分菌。最后进行详细的排查分析，才有可能确定主要污染源和关键污染菌。
根据国外的报道显示，种蛋的污染主要是在孵化过程中大肠杆菌的污染，而种鸡场对种蛋的污染很小。但是，到目前为止国内相关的报道不多，且实验设计不够合理。因此，国内种蛋污染或许与二者都有关系，但需要进一步的研究。
Cortés CR, Téllez Isaías G, López Cuello C, Villaseca-Flores JM, Anderson RC, Eslava Campos C. Bacterial isolation rate from fertile eggs, hatching eggs, and neonatal broilers with yolk sac infection. Rev Latinoam Microbiol. 2004;46(1-2):12-16.
文章摘要
Yolk sac infection (YSI) is a major cause of mortality of broilers during the first week post-hatching. The aim of the present-study was to analyze the possible sources of fertile egg contamination and to establish the etiology of YSI. Sixty fertile eggs, sixty sawdust samples from the nest, sixty nonfertile 19 to 21 day old incubation eggs and liver and yolk sac samples from 216 dead, 1 to 7 day old chicks, were cultured. Five hundred and eighty eight colonies were isolated and further characterized using biochemical tests. Escherichia coli was the most common bacterium recovered from all samples except the sawdust and fertile eggs collected from the nest. Fertile egg contamination at breeder farm level was found to be minimal. In broilers, both mortality and the rate of E. coli isolation were increased with the time. These results suggest that egg contamination does not occur at the breeders farm, as previously has been reported. Bacterial contamination causing YSI in vertically integrated operations can occur at a latter stage. It can be considered that the main etiologic agent of YSI is E. coli, since YSI mortality was highly correlated with E. coli isolation. 很多年以前，我去过美国罗斯育种公司种鸡场，他们的种蛋在入孵前不消毒。他们说，数据证明，入孵前消毒与不消毒结果是一样的。既然没效果，为什么还要去费事又费钱呢？
当时我们只能目瞪口呆。 tsmc 发表于 2021-11-05 10:49
常老师你好，我觉得你这样设计实验的话，实验结果很难说明问题。
要想分析受精蛋可能的污染问题（或者是造成死胚和弱雏的原因），需要对受精蛋和未受精蛋同时进行孵化处理，在19-21天内进行采样分菌，另外也要对鸡舍污染物（如粪便）、死胚、孵化出来1-7内死亡雏鸡的肝脏和卵黄囊进行采样分菌。最后进行详细的排查分析，才有可能确定主要污染源和关键污染菌。
根据国外的报道显示，种蛋的污染主要是在孵化过程中大肠杆菌的污染，而种鸡场对种蛋的污染很小。但是，到目前为止国内相关的报道不多，且实验设计不够合理。因此，国内种蛋污染或许与二者都有关系，但需要进一步的研究。
Cortés CR, Téllez Isaías G, López Cuello C, Villaseca-Flores JM, Anderson RC, Eslava Campos C. Bacterial isolation rate from fertile eggs, hatching eggs, and neonatal broilers with yolk sac infection. Rev Latinoam Microbiol. 2004;46(1-2):12-16.
文章摘要
Yolk sac infection (YSI) is a major cause of mortality of broilers during the first week post-hatching. The aim of the present-study was to analyze the possible sources of fertile egg contamination and to establish the etiology of YSI. Sixty fertile eggs, sixty sawdust samples from the nest, sixty nonfertile 19 to 21 day old incubation eggs and liver and yolk sac samples from 216 dead, 1 to 7 day old chicks, were cultured. Five hundred and eighty eight colonies were isolated and further characterized using biochemical tests. Escherichia coli was the most common bacterium recovered from all samples except the sawdust and fertile eggs collected from the nest. Fertile egg contamination at breeder farm level was found to be minimal. In broilers, both mortality and the rate of E. coli isolation were increased with the time. These results suggest that egg contamination does not occur at the breeders farm, as previously has been reported. Bacterial contamination causing YSI in vertically integrated operations can occur at a latter stage. It can be considered that the main etiologic agent of YSI is E. coli, since YSI mortality was highly correlated with E. coli isolation.
既然没有国内的资料。补充点数据不是很好吗！ 中兽 发表于 2021-11-03 23:54
有点废话了，鸡蛋里面这么多抗体和溶菌酶。除非那个鸡蛋不正常，因为不正常所以也发育不了。
有兴趣的话，看看家禽科学上我发表的论文《山东省肉杂鸡种蛋带菌情况调查与防治措施》。有网友在本网上转载过。
http://magimg.jbzyw.com/pic/20211105/1636120674819267_807.jpg yuleicn 发表于 2021-11-04 16:39
您肯定也分出过沙门氏菌吧
商品蛋鸡很少分离到了。 常老师1 发表于 2021-11-05 21:58
有兴趣的话，看看家禽科学上我发表的论文《山东省肉杂鸡种蛋带菌情况调查与防治措施》。有网友在本网上转载过。
OK 没事游山看水吧
http://magimg.jbzyw.com/pic/20211106/1636187749228332_316.jpg
http://magimg.jbzyw.com/pic/20211106/1636187816797542_363.jpg
http://magimg.jbzyw.com/pic/20211106/1636187862515244_916.jpg 马连松 发表于 2021-11-06 16:38
没事游山看水吧
啥时候来泰安了？
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